Skip to Main content Skip to Navigation
Journal articles

Positive contrast high-resolution 3D-cine imaging of the cardiovascular system in small animals using a UTE sequence and iron nanoparticles at 4.7, 7 and 9.4 T

Abstract : Background: To show that 3D sequences with ultra-short echo times (UTEs) can generate a positive contrast whatever the magnetic field (4.7, 7 or 9.4 T) and whatever Ultra Small Particles of Iron Oxide (USPIO) concentration injected and to use it for 3D time-resolved imaging of the murine cardiovascular system with high spatial and temporal resolutions. Methods: Three different concentrations (50, 200 and 500 μmol Fe/kg) of USPIO were injected in mice and static images of the middle part of the animals were acquired at 4.7, 7 and 9.4 T pre and post-contrast with UTE (TE/TR = 0.05/4.5 ms) sequences. Signal-to-Noise Ratio (SNR) and Contrast-to-Noise Ratio (CNR) of blood and static tissus were evaluated before and after contrast agent injection. 3D-cine images (TE/TR = 0.05/3.5 ms, scan time < 12 min) at 156 μm isotropic resolution of the mouse cardiopulmonary system were acquired prospectively with the UTE sequence for the three magnetic fields and with an USPIO dose of 200 μmol Fe/kg. SNR, CNR and signal homogeneity of blood were measured. High spatial (104 μm) or temporal (3.5 ms) resolution 3D-cine imaging (scan time < 35 min) isotropic resolution were also performed at 7 T with a new sequence encoding scheme. Results: UTE imaging generated positive contrast and higher SNR and CNR whatever the magnetic field and the USPIO concentration used compared to pre-contrast images. Time-resolved 3D acquisition enables high blood SNR (66.6 ± 4.5 at 7 T) and CNR (33.2 ± 4.2 at 7 T) without flow or motion artefact. Coronary arteries and aortic valve were visible on images acquired at 104 μm resolution. Conclusions: We have demonstrated that by combining the injection of iron nanoparticles with 3D-cine UTE sequences, it was possible to generate a strong positive contrast between blood and surrounding tissues. These properties were exploited to produce images of the cardiovascular system in small animals at high magnetic fields with a high spatial and temporal resolution. This approach might be useful to measure the functional cardiac parameters or to assess anatomical modifications to the blood vessels in cardio-vascular disease models.
Document type :
Journal articles
Complete list of metadatas

Cited literature [44 references]  Display  Hide  Download

https://hal-cnrs.archives-ouvertes.fr/hal-02486814
Contributor : Karine Viaud <>
Submitted on : Friday, February 21, 2020 - 11:18:12 AM
Last modification on : Friday, February 21, 2020 - 2:23:43 PM
Long-term archiving on: : Friday, May 22, 2020 - 4:34:15 PM

File

12968_2015_Article_167.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Aurélien Trotier, William Lefrançois, Kris van Renterghem, Jean-Michel Franconi, Eric Thiaudière, et al.. Positive contrast high-resolution 3D-cine imaging of the cardiovascular system in small animals using a UTE sequence and iron nanoparticles at 4.7, 7 and 9.4 T. Journal of Cardiovascular Magnetic Resonance, BioMed Central, 2015, 17, pp.53. ⟨10.1186/s12968-015-0167-4⟩. ⟨hal-02486814⟩

Share

Metrics

Record views

82

Files downloads

114