Neutrophil Elastase Activity Imaging: Recent Approaches in the Design and Applications of Activity-Based Probes and Substrate-Based Probes

Natacha Jugniot 1 Pierre Voisin 1 Abderrazzak Bentaher 2 Philippe Mellet 1
1 RMSB - Résonance magnétique des systèmes biologiques
2 PI3 - Physiopathologie de l'immunodépression associée aux réponses inflammatoires systémiques - EA 7426
Abstract : The last few decades of protease research has con rmed that a number of important biological processes are strictly dependent on proteolysis. Neutrophil elastase (NE) is a critical protease in immune response and host defense mechanisms in both physiological and disease-associated conditions. Particularly, NE has been identi ed as a promising biomarker for early diagnosis of lung in ammation. Recent studies have shown an increasing interest in developing methods for NE activity imaging both in vitro and in vivo. Unlike anatomical imaging modalities, functional molecular imaging, including enzymatic activities, enables disease detection at a very early stage and thus constitutes a much more accurate approach. When combined with advanced imaging technologies, opportunities arise for measuring imbalanced proteolytic activities with unprecedented details. Such technologies consist in building the highest resolved and sensitive instruments as well as the most speci c probes based either on peptide substrates or on covalent inhibitors. is review outlines strengths and weaknesses of these technologies and discuss their applications to investigate NE activity as biomarker of pulmonary in ammatory diseases by imaging.
Document type :
Journal articles
Complete list of metadatas

Cited literature [101 references]  Display  Hide  Download
https://hal-cnrs.archives-ouvertes.fr/hal-02472427
Contributor : Karine Viaud <>
Submitted on : Monday, February 10, 2020 - 11:26:39 AM
Last modification on : Wednesday, February 12, 2020 - 1:47:14 AM

File

1ed794dce0d622c4d4f0cd9ae1c6b0...
Publisher files allowed on an open archive

Identifiers

Collections

CNRS | UNIV-LYON1 | UDL

Citation

Natacha Jugniot, Pierre Voisin, Abderrazzak Bentaher, Philippe Mellet. Neutrophil Elastase Activity Imaging: Recent Approaches in the Design and Applications of Activity-Based Probes and Substrate-Based Probes. Contrast Media and Molecular Imaging, Wiley, 2019, 2019, pp.7417192. ⟨10.1155/2019/7417192⟩. ⟨hal-02472427⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

3

Files downloads

5

Contact

Informations

CCSD