HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

A gene-based map of the Nod factor-independent Aeschynomene evenia sheds new light on the evolution of nodulation and legume genomes

Abstract : Aeschynomene evenia has emerged as a new model legume for the deciphering of the molecular mechanisms of an alternative symbiotic process that is independent of the Nod factors. Whereas most of the research on nitrogen-fixing symbiosis, legume genetics and genomics has so far fo-cused on Galegoid and Phaseolid legumes, A. evenia falls in the more basal and understudied Dalbergioid clade along with peanut (Arachis hypogaea). To provide insights into the symbiotic genes content and the structure of the A. evenia genome, we established a gene-based genetic map for this species. Firstly, an RNAseq analysis was performed on the two parental lines selected to generate a F 2 mapping population. The transcriptomic data were used to develop molecular markers and they allowed the identification of most symbiotic genes. The resulting map comprised 364 markers arranged in 10 linkage groups (2n ¼ 20). A comparative analysis with the sequenced genomes of Arachis duranensis and A. ipaensis, the diploid ancestors of peanut, indicated blocks of conserved macrosynteny. Altogether, these results provided important clues regarding the evolution of symbiotic genes in a Nod factor-independent context. They provide a Downloaded from https://academic.oup.com/dnaresearch/article-abstract/23/4/365/2469965 by BIU Montpellier user on 12 March 2020 basis for a genome sequencing project and pave the way for forward genetic analysis of symbio-sis in A. evenia.
Document type :
Journal articles
Complete list of metadata

Cited literature [48 references]  Display  Hide  Download

Contributor : Pascal Ratet Connect in order to contact the contributor
Submitted on : Thursday, March 12, 2020 - 1:12:04 PM
Last modification on : Wednesday, May 25, 2022 - 1:27:09 PM
Long-term archiving on: : Saturday, June 13, 2020 - 3:31:10 PM


Publisher files allowed on an open archive


Distributed under a Creative Commons Attribution - NonCommercial 4.0 International License



Clémence Chaintreuil, Ronan Rivallan, David Bertioli, Christophe Klopp, Jerome Gouzy, et al.. A gene-based map of the Nod factor-independent Aeschynomene evenia sheds new light on the evolution of nodulation and legume genomes. DNA Research, Oxford University Press (OUP), 2016, 23 (4), pp.365-376. ⟨10.1093/dnares/dsw020⟩. ⟨hal-02410329⟩



Record views


Files downloads