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Journal articles
The complete genome sequence of Ensifer meliloti strain CCMM B554 (FSM-MA), a highly effective nitrogen-fixing microsymbiont of Medicago truncatula Gaertn
Marianna Nagymihaly
1, 2
Bálint Vásarhelyi
Quentin Barrière
1, 2
Teik-Min Chong
3
Balázs Bálint
Péter Bihari
Kar-Wai Hong
3
Balázs Horváth
Jamal Ibijbijen
4
Mohammed Amar
Attila Farkas
5
Eva Kondorosi
Kok-Gan Chan
3
Véronique Gruber
6
Pascal Ratet
6
Peter Mergaert
1, 2
Attila Kereszt
Marianna Nagymihaly 1, 2
Author
Bálint Vásarhelyi
Author
Quentin Barrière 1, 2
Author
Teik-Min Chong 3
Author
Kok-Gan Chan 3
Author
Véronique Gruber 6
Author
Pascal Ratet 6
Author IdHAL : pascal-ratet ORCID : https://orcid.org/0000-0002-8621-1495
Peter Mergaert 1, 2
Author IdHAL : peter-mergaert ORCID : https://orcid.org/0000-0002-5919-7317
Attila Kereszt
Author
1
I2BC - Institut de Biologie Intégrative de la Cellule (Bâtiment 21, 1 avenue de la Terrasse, 91198 Gif/Yvette cedex - France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives : DRF/I2BC (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
- Université Paris-Saclay (Espace Technologique, Bat. Discovery - RD 128 - 2e ét., 91190 Saint-Aubin - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 9198 (France)
2
PBI - Intéractions Plantes-Bactéries (I2BC, Bâtiment 21, 1 avenue de la Terrasse, 91198 Gif/Yvette cedex - France)
- Dpt Microbio - Département Microbiologie (I2BC, Bâtiment 21, 1 avenue de la Terrasse, 91198 Gif/Yvette cedex - France)
- I2BC - Institut de Biologie Intégrative de la Cellule (Bâtiment 21, 1 avenue de la Terrasse, 91198 Gif/Yvette cedex - France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives : DRF/I2BC (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
- Université Paris-Saclay (Espace Technologique, Bat. Discovery - RD 128 - 2e ét., 91190 Saint-Aubin - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 9198 (France)
- I2BC - Institut de Biologie Intégrative de la Cellule (Bâtiment 21, 1 avenue de la Terrasse, 91198 Gif/Yvette cedex - France)
3
Institute of Biological Sciences (Faculty of Science, Universiti Malaya, Kuala Lumpur - Malaysia)
- Universiti Malaya (France)
4
Faculté des Sciences (France)
- UNIGE - Université de Genève (24 rue du Général-Dufour CH - 1211 Genève 4 - Switzerland)
5
Inst Plant Genom Human Biotechnol & Bioenergy (Hungary)
6
IPS2 (UMR_9213 / UMR_1403) - Institut des Sciences des Plantes de Paris-Saclay (Institute of Plant Sciences - Paris-Saclay
Bâtiment 630, rue de Noetzlin
Plateau du Moulon
CS80004
91192 - Gif-sur-Yvette Cedex - France)
- INRA - Institut National de la Recherche Agronomique : UMR_1403 (France)
- UP11 - Université Paris-Sud - Paris 11 (Bâtiment 300 - 91405 Orsay cedex - France)
- UPD7 - Université Paris Diderot - Paris 7 (5 rue Thomas-Mann - 75205 Paris cedex 13 - France)
- UEVE - Université d'Évry-Val-d'Essonne (Boulevard François Mitterrand 91025 Evry Cedex - France)
- CNRS - Centre National de la Recherche Scientifique : UMR_9213 (France)
Abstract : Strain CCMM B554, also known as FSM-MA, is a soil dwelling and nodule forming, nitrogen-fixing bacterium isolated from the nodules of the legume Medicago arborea L. in the Maamora Forest, Morocco. The strain forms effective nitrogen fixing nodules on species of the Medicago, Melilotus and Trigonella genera and is exceptional because it is a highly effective symbiotic partner of the two most widely used accessions, A17 and R108, of the model legume Medicago truncatula Gaertn. Based on 16S rRNA gene sequence, multilocus sequence and average nucleotide identity analyses, FSM-MA is identified as a new Ensifer meliloti strain. The genome is 6,70 Mbp and is comprised of the chromosome (3,64 Mbp) harboring 3574 predicted genes and two megaplasmids, pSymA (1,42 Mbp) and pSymB (1,64 Mbp) with respectively 1481 and 1595 predicted genes. The average GC content of the genome is 61.93%. The FSM-MA genome structure is highly similar and co-linear to other E. meliloti strains in the chromosome and the pSymB megaplasmid while, in contrast, it shows high variability in the pSymA plasmid. The large number of strain-specific sequences in pSymA as well as strain-specific genes on pSymB involved in the biosynthesis of the lipopolysaccharide and capsular polysaccharide surface polysaccharides may encode novel symbiotic functions explaining the high symbiotic performance of FSM-MA.
Document type :
Journal articles
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https://hal-cnrs.archives-ouvertes.fr/hal-02410310
Contributor : Pascal Ratet Connect in order to contact the contributor
Submitted on : Friday, December 13, 2019 - 5:58:26 PM
Last modification on : Tuesday, October 12, 2021 - 3:06:03 PM
Contributor : Pascal Ratet Connect in order to contact the contributor
Submitted on : Friday, December 13, 2019 - 5:58:26 PM
Last modification on : Tuesday, October 12, 2021 - 3:06:03 PM
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- HAL Id : hal-02410310, version 1
- DOI : 10.1186/s40793-017-0298-3
- PUBMED : 29255570
- PUBMEDCENTRAL : PMC5729237
Collections
CNRS | INRA | CEA | CEA-UPSAY | I2BC | CEA-DRF | UNIV-EVRY | UNIV-PARIS7 | USPC | JOLIOT | IPS2 | INRAE | UNIV-PARIS | UP-SCIENCES | GS-ENGINEERING | GS-BIOSPHERA | INSTITUT-SCIENCES-LUMIERE | GS-HEALTH-DRUG-SCIENCES
Citation
Marianna Nagymihaly, Bálint Vásarhelyi, Quentin Barrière, Teik-Min Chong, Balázs Bálint, et al.. The complete genome sequence of Ensifer meliloti strain CCMM B554 (FSM-MA), a highly effective nitrogen-fixing microsymbiont of Medicago truncatula Gaertn. Standards in Genomic Sciences, BioMedCentral, 2017, 12, ⟨10.1186/s40793-017-0298-3⟩. ⟨hal-02410310⟩
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