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Journal articles
3D anatomical and perfusion MRI for longitudinal evaluation of biomaterials for bone regeneration of femoral bone defect in rats
Abstract : Magnetic Resonance Imaging (MRI) appears as a good surrogate to Computed Tomography (CT) scan as it does not involve radiation. In this context, a 3D anatomical and perfusion MR imaging protocol was developed to follow the evolution of bone regeneration and the neo-vascularization in femoral bone defects in rats. For this, three different biomaterials based on Pullulan-Dextran and containing either Fucoidan or HydroxyApatite or both were implanted. In vivo MRI, ex vivo micro-CT and histology were performed 1, 3 and 5 weeks after implantation. The high spatially resolved (156 × 182 × 195 µm) anatomical images showed a high contrast from the defects filled with biomaterials that decreased over time due to bone formation. The 3D Dynamic Contrast Enhanced (DCE) imaging with high temporal resolution (1 image/19 s) enabled to detect a modification in the Area-Under-The-Gadolinium-Curve over the weeks post implantation. The high sensitivity of MRI enabled to distinguish which biomaterial was the least efficient for bone regeneration, which was confirmed by micro-CT images and by a lower vessel density observed by histology. In conclusion, the methodology developed here highlights the efficiency of longitudinal MRI for tissue engineering as a routine small animal exam.
Cited literature [35 references]
https://hal-cnrs.archives-ouvertes.fr/hal-02374397
Contributor : Karine Viaud Connect in order to contact the contributor
Submitted on : Thursday, November 21, 2019 - 3:05:59 PM
Last modification on : Monday, March 28, 2022 - 10:34:02 AM
Contributor : Karine Viaud Connect in order to contact the contributor
Submitted on : Thursday, November 21, 2019 - 3:05:59 PM
Last modification on : Monday, March 28, 2022 - 10:34:02 AM
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