3D anatomical and perfusion MRI for longitudinal evaluation of biomaterials for bone regeneration of femoral bone defect in rats

Emeline Ribot 1 Clément Tournier 2 Rachida Aid-Launais 3 Neha Koonjoo 1 Hugo Oliveira 4 Aurelien Trotier Sylvie Rey 5 Didier Wecker 6 Didier Letourneur 3 Joëlle Amedee Vilamitjana 4 Sylvain Miraux 1
1 RMSB - Résonance magnétique des systèmes biologiques
2 UFR des Sciences Médicales - Faculté de Médecine H. Bastaraud
3 LVTS (UMR_S_1148 / U1148) - Laboratoire de Recherche Vasculaire Translationnelle
4 BIOTIS - Bioingénierie tissulaire
5 Equipe avenir "Environmental Epidemiology Applied to Fecundity and Reproduction"
6 Bruker BioSpin MRI GmbH [Ettlingen, Germany]
Abstract : Magnetic Resonance Imaging (MRI) appears as a good surrogate to Computed Tomography (CT) scan as it does not involve radiation. In this context, a 3D anatomical and perfusion MR imaging protocol was developed to follow the evolution of bone regeneration and the neo-vascularization in femoral bone defects in rats. For this, three different biomaterials based on Pullulan-Dextran and containing either Fucoidan or HydroxyApatite or both were implanted. In vivo MRI, ex vivo micro-CT and histology were performed 1, 3 and 5 weeks after implantation. The high spatially resolved (156 × 182 × 195 µm) anatomical images showed a high contrast from the defects filled with biomaterials that decreased over time due to bone formation. The 3D Dynamic Contrast Enhanced (DCE) imaging with high temporal resolution (1 image/19 s) enabled to detect a modification in the Area-Under-The-Gadolinium-Curve over the weeks post implantation. The high sensitivity of MRI enabled to distinguish which biomaterial was the least efficient for bone regeneration, which was confirmed by micro-CT images and by a lower vessel density observed by histology. In conclusion, the methodology developed here highlights the efficiency of longitudinal MRI for tissue engineering as a routine small animal exam.
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Emeline Ribot, Clément Tournier, Rachida Aid-Launais, Neha Koonjoo, Hugo Oliveira, et al.. 3D anatomical and perfusion MRI for longitudinal evaluation of biomaterials for bone regeneration of femoral bone defect in rats. Scientific Reports, Nature Publishing Group, 2017, 7 (1), ⟨10.1038/s41598-017-06258-0⟩. ⟨hal-02374397⟩

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