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HAL-CNRSCNRS scientific publications |
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Journal articles
IL-33 signalling in liver immune cells enhances drug-induced liver injury and inflammation
Abstract : The aim of this study was to investigate the contribution of IL-33/ST2 axis in the onset and progression of acute liver injury using a mice model of drug-induced liver injury (DILI). DILI was induced by overdose administration of acetaminophen (APAP) by oral gavage in wild-type BALB/c, ST2-deficient mice and in different bone marrow chimeras. Neutrophils were depleted by anti-Ly6G and macrophages with clodronate liposomes (CLL). Blood and liver were collected for biochemical, immunologic and genetic analyses. Mice were imaged by confocal intravital microscopy and liver non-parenchymal cells and hepatocytes were isolated for flow cytometry, genetic and immunofluorescence studies. Acetaminophen overdose caused a massive necrosis and accumulation of immune cells within the liver, concomitantly with IL-33 and chemokine release. Liver non-parenchymal cells were the major sensors for IL-33, and amongst them, neutrophils were the major players in amplification of the inflammatory response triggered by IL-33/ST2 signalling pathway. Blockage of IL-33/ST2 axis reduces APAP-mediated organ injury by dampening liver chemokine release and activation of resident and infiltrating liver non-parenchymal cells.
https://hal-cnrs.archives-ouvertes.fr/hal-02126492
Contributor : Valérie Quesniaux Connect in order to contact the contributor
Submitted on : Saturday, May 11, 2019 - 6:04:45 PM
Last modification on : Wednesday, October 27, 2021 - 7:09:44 AM
Contributor : Valérie Quesniaux Connect in order to contact the contributor
Submitted on : Saturday, May 11, 2019 - 6:04:45 PM
Last modification on : Wednesday, October 27, 2021 - 7:09:44 AM
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