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Rapid and accurate detection of Escherichia coli growth by fluorescent pH-sensitive organic nanoparticles for high-throughput screening applications.

Yang Si 1 Chloé Grazon 1 Gilles Clavier 1 Rieger Jutta 2 J.F. Audibert 1 Bianca Sclavi 3 Rachel Méallet-Renault 1
2 LCP - Chimie des polymères
UPMC - Université Pierre et Marie Curie - Paris 6, IPCM - Institut Parisien de Chimie Moléculaire, CNRS - Centre National de la Recherche Scientifique : UMR7610
Abstract : Rapid detection of bacterial growth is an important issue in the food industry and for medical research. Here we present a novel kind of pH-sensitive fluorescent nanoparticles (FANPs) that can be used for the rapid and accurate real-time detection of Escherichia coli growth. These organic particles are designed to be non-toxic and highly water-soluble. Here we show that the coupling of pH sensitive fluoresceinamine to the nanoparticles results in an increased sensitivity to changes in pH within a physiologically relevant range that can be used to monitor the presence of live bacteria. In addition, these FANPs do not influence bacterial growth and are stable over several hours in a complex medium and in the presence of bacteria. The use of these FANPs allows for continuous monitoring of bacterial growth via real-time detection over long time scales in small volumes and can thus be used for the screening of a large number of samples for high-throughput applications such as screening for the presence of antibiotic resistant strains.
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https://hal.archives-ouvertes.fr/hal-01230475
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Submitted on : Monday, October 25, 2021 - 11:03:35 AM
Last modification on : Tuesday, November 16, 2021 - 5:26:17 AM

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Yang Si, Chloé Grazon, Gilles Clavier, Rieger Jutta, J.F. Audibert, et al.. Rapid and accurate detection of Escherichia coli growth by fluorescent pH-sensitive organic nanoparticles for high-throughput screening applications.. Biosensors and Bioelectronics, Elsevier, 2016, 75, pp.320-327. ⟨10.1016/j.bios.2015.08.028⟩. ⟨hal-01230475⟩

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