FRET-mediated quenching of BODIPY fluorescent nanoparticles by methylene blue and its application to bacterial imaging - Archive ouverte HAL Access content directly
Journal Articles Photochemical & Photobiological Sciences Year : 2022

FRET-mediated quenching of BODIPY fluorescent nanoparticles by methylene blue and its application to bacterial imaging

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Abstract

High resolution and good signal to noise ratio is a requirement in cell imaging. However, after labelling with fluorescent entities, and besides several washing steps, there is often an unwanted fluorescent background that reduces the images resolution. For this purpose, we developed an approach to remove the signal from extra-cellular fluorescent nanoparticles (FNPs) during bacteria imaging, without any washing steps. Our idea is to use methylene blue to quench (>90%) the emission of BODIPY-based fluorescent polymer nanoparticle by a FRET process. This "Hide-and-Seek Game" approach offers a novel strategy to apply fluorescence quenching in bioimaging to improve image accuracy.
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Dates and versions

hal-03690556 , version 1 (08-06-2022)

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Yang Si, Chloé Grazon, Gilles Clavier, Jean-Frédéric Audibert, Bianca Sclavi, et al.. FRET-mediated quenching of BODIPY fluorescent nanoparticles by methylene blue and its application to bacterial imaging. Photochemical & Photobiological Sciences , 2022, ⟨10.1007/s43630-022-00215-1⟩. ⟨hal-03690556⟩
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