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Article Dans Une Revue BMC Research Notes Année : 2020

Plant and mouse EB1 proteins have opposite intrinsic properties on the dynamic instability of microtubules

Résumé

Objective: Most eukaryotic cells contain microtubule filaments, which play central roles in intra-cellular organization. However, microtubule networks have a wide variety of architectures from one cell type and organism to another. Nonetheless, the sequences of tubulins, of Microtubule Associated proteins (MAPs) and the structure of microtubules are usually well conserved throughout the evolution. MAPs being known to be responsible for regulating microtubule organization and dynamics, this raises the question of the conservation of their intrinsic properties. Indeed, knowing how the intrinsic properties of individual MAPs differ between organisms might enlighten our understanding of how distinct microtubule networks are built. End-Binding protein 1 (EB1), first described as a MAP in yeast, is conserved in plants and mammals. The intrinsic properties of the mammalian and the yeast EB1 proteins have been well described in the literature but, to our knowledge, the intrinsic properties of EB1 from plant and mammals have not been compared thus far. Results: Here, using an in vitro assay, we discovered that plant and mammalian EB1 purified proteins have different intrinsic properties on microtubule dynamics. Indeed, the mammalian EB1 protein increases microtubules dynamic while the plant EB1 protein stabilizes them.
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hal-03010752 , version 1 (24-11-2020)

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Arthur Molines, Virginie Stoppin-Mellet, Isabelle Arnal, Frédéric Coquelle. Plant and mouse EB1 proteins have opposite intrinsic properties on the dynamic instability of microtubules. BMC Research Notes, 2020, 13 (1), ⟨10.1186/s13104-020-05139-6⟩. ⟨hal-03010752⟩
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