Reconstruction of functional human epidermis equivalent containing 5%IPS-derived keratinocytes treated with mitochondrial stimulating plant extracts - Archive ouverte HAL Access content directly
Journal Articles Scientific Reports Year : 2022

Reconstruction of functional human epidermis equivalent containing 5%IPS-derived keratinocytes treated with mitochondrial stimulating plant extracts

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Marielle Moreau
  • Function : Author
Christophe Capallere
  • Function : Author
Christelle Plaza
  • Function : Author
Céline Meyrignac
  • Function : Author
Karl Pays
  • Function : Author
Bruno Bavouzet
  • Function : Author
Jean-Marie Botto
  • Function : Author
Carine Nizard
  • Function : Author
Anne-Laure Bulteau

Abstract

Abstract Reconstructed human epidermis equivalents (RHE) have been developed as a clinical skin substitute and as the replacement for animal testing in both research and industry. KiPS, or keratinocytes derived from induced pluripotent stem cells (iPSCs) are frequently used to generate RHE. In this study, we focus on the mitochondrial performance of the KiPS derived from iPSCs obtained from two donors. We found that the KiPS derived from the older donor have more defective mitochondria. Treatment of these KiPS with a plant extract enriched in compounds known to protect mitochondria improved mitochondrial respiration and rendered them fully competent to derive high-quality RHE. Overall, our results suggest that improving mitochondrial function in KiPS is one of the key aspects to obtain a functional RHE and that our plant extracts can improve in this process.
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Dates and versions

hal-03830642 , version 1 (10-11-2022)

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Marielle Moreau, Christophe Capallere, Laurent Chavatte, Christelle Plaza, Céline Meyrignac, et al.. Reconstruction of functional human epidermis equivalent containing 5%IPS-derived keratinocytes treated with mitochondrial stimulating plant extracts. Scientific Reports, 2022, 12 (1), pp.9073. ⟨10.1038/s41598-022-13191-4⟩. ⟨hal-03830642⟩
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