Influenza A virus RNA Polymerase targets the chromatin of innate immune response genes - Archive ouverte HAL Access content directly
Preprints, Working Papers, ... Year :

Influenza A virus RNA Polymerase targets the chromatin of innate immune response genes

(1) , (2) , (1) , (2) , (2) , (1) , (1)
1
2

Abstract

Abstract The influenza A virus (IAV) profoundly affects host cell nuclear processes to accommodate for its transcription. In particular, the IAV RNA polymerase (FluPol) associates with host genes to snatch capped 5’-ends from nascent messenger RNAs, that are then used to prime viral transcription. The extend of FluPol-host gene interaction and its actual consequence on host transcription are still poorly characterized. We show here, using genome-wide approaches, that the PA, PB1, and PB2 subunits of FluPol establish contacts with both chromatin and RNA transcripts at promoters, but also within the coding region of genes. These interactions also reach into downstream regions where recruitment of FluPol subunits correlates with previously reported transcription termination defects induced by IAV, indicative of an implication of the FluPol in this viral strategy to limit host cell defense by interfering with transcription re-initiation. The latter is further suggested by a bias in the FluPol genomic targets, enriched in genes associated with anti-viral defense. Together, our observations suggest that FluPol-chromatin binding contributes to targeted dampening of host immune response pathways.
Fichier principal
Vignette du fichier
2022.03.30.486363v1.full.pdf (1.29 Mo) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

hal-03767079 , version 1 (01-09-2022)

Identifiers

Cite

Jia Yi, Jessica Morel, Mickaël Costallat, Nathalie Lejal, Bernard Delmas, et al.. Influenza A virus RNA Polymerase targets the chromatin of innate immune response genes. 2022. ⟨hal-03767079⟩
15 View
5 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More